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Illustrative <t>SEC‐HPLC</t> (A) and SDS‐PAGE (B) analysis of IgG (11 mg mL −1 ) feed and permeate samples following centrifugal ultrafiltration at 3000 rpm; Total protein biuret assay (C) recovery of IgG (11 mg mL −1 ) following centrifugal ultrafiltration using 33 µm virus removal filter paper.
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Illustrative <t>SEC‐HPLC</t> (A) and SDS‐PAGE (B) analysis of IgG (11 mg mL −1 ) feed and permeate samples following centrifugal ultrafiltration at 3000 rpm; Total protein biuret assay (C) recovery of IgG (11 mg mL −1 ) following centrifugal ultrafiltration using 33 µm virus removal filter paper.
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Illustrative SEC‐HPLC (A) and SDS‐PAGE (B) analysis of IgG (11 mg mL −1 ) feed and permeate samples following centrifugal ultrafiltration at 3000 rpm; Total protein biuret assay (C) recovery of IgG (11 mg mL −1 ) following centrifugal ultrafiltration using 33 µm virus removal filter paper.

Journal: Biotechnology Journal

Article Title: Analytical Centrifugal Ultrafiltration as a Tool for High Throughput Process Development in Virus Removal Filtration

doi: 10.1002/biot.70227

Figure Lengend Snippet: Illustrative SEC‐HPLC (A) and SDS‐PAGE (B) analysis of IgG (11 mg mL −1 ) feed and permeate samples following centrifugal ultrafiltration at 3000 rpm; Total protein biuret assay (C) recovery of IgG (11 mg mL −1 ) following centrifugal ultrafiltration using 33 µm virus removal filter paper.

Article Snippet: A Hitachi Chromaster HPLC system (Hitachi High‐Tech, Japan) equipped with a 5110 Pump (operating flow rate range 0.001 to 9.999 mL/min), Auto Sampler 5260 (Withstand pressure 60 MPa, Loop injection method), and a 6310 Column Oven, vertical type was used.

Techniques: SDS Page, Virus